Establishment of indirect immunofluorescence assay for rotavirus
نویسندگان
چکیده
منابع مشابه
Typing Haemophilus ducreyi by indirect immunofluorescence assay.
Rabbits were inoculated with untreated whole cells of 16 strains of Haemophilus ducreyi. Homologous titres in an indirect immunofluorescence test ranged from 1/320 to 1/1280. No cross reactions, or very few, were observed between antisera to these strains and 22 other (possible taxonomically related) species, strains, and genera. A large diversity in types of antigen was observed among the stra...
متن کاملSerological detection of Capillaria hepatica by indirect immunofluorescence assay.
In this paper, a serological assay for the detection of antibodies to Capillaria hepatica, a zoonotic parasite, is described. In the past, the only way of detecting Capillaria hepatica was to perform a liver biopsy. The indirect immunofluorescence (IIF) assay, based on liver sections of naturally infected mice and human serum samples, is suitable for detecting early stages of human infections a...
متن کاملValidation of Legionella pneumophila indirect immunofluorescence assay with epidemic sera.
Sera from six outbreaks of legionellosis and four outbreaks of pneumonia of other etiologies were tested with the indirect immunofluorescence assay (IFA) as currently performed. The current IFA is at least as sensitive as the original test in detecting cases of Legionnaires disease (78 to 91%). By using Center for Disease Control criteria for a positive (fourfold increase in titer during conval...
متن کاملComparison of enzyme-linked immunosorbent assay and indirect immunofluorescence assay in the diagnosis of human strongyloidiasis.
BACKGROUND An enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence assay (IFA) were evaluated for serological diagnosis of human strongyloidiasis. METHODS Serum specimens obtained from 46 individuals infected with Strongyloides stercoralis, 37 healthy persons and 381 persons with other parasitic infections were tested using an IgG-ELISA that used crude antigen of S. sterc...
متن کاملOptimal Cutoff Titers for Indirect Immunofluorescence Assay for Diagnosis of Scrub Typhus
We determined the optimal cutoff titers in admission and convalescent-phase samples for scrub typhus indirect immunofluorescence assay using Bayesian latent class models. Cutoff titers of ≥1:3,200 in an admission sample or of a ≥4-fold rise to ≥1:3,200 in a convalescent-phase sample provided the highest accuracy (sensitivity, 81.6%; specificity, 100%).
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ژورنال
عنوان ژورنال: Acta virologica
سال: 2016
ISSN: 1336-2305
DOI: 10.4149/av_2016_01_78